Enamel and dentine remineralization by nano-hydroxyapatite toothpastes
Specimens were demineralized, randomly divided into five groups, and exposed to an aqueous remineralizing solution for two and five weeks (37 8C). Brushing procedures were performed with the respective toothpaste/storage solution slurry twice daily (2 5 s; total contact time of the slurries 2 120 s/d): storage in remineralizing solution only (0); additional brushing with B (20 wt% zinc carbonate nano-hydroxyapatite, ZnCO3/n-HAp); BS (24 wt% ZnCO3/n-HAp); E (0.14 wt% amine fluoride); or A (7 wt% pure n-HAp). Differences in mineral loss (DDZ) before and after storage/treatment were microradiographically evaluated.
